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Home Antibody All anti-PDF antibodies

Anti-PDF TRUEMAB Antibody Clone OTI3F3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502988 PDF mouse monoclonal antibody, clone OTI3F3 (formerly 3F3) 100ul $379 In Stock
LC411712 PDF HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF502988 Carrier-free (BSA/glycerol-free) PDF mouse monoclonal antibody, clone OTI3F3 (formerly 3F3) 100ug $450 3-4 weeks

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(2)
IF(1)
FC(3)

OriGene Data

ImmunogenFull length human recombinant protein of human PDF (NP_071736) produced in E.coli.
Clone NameClone OTI3F3 IsotypeIgG2b
Species ReactivityHuman Concentration0.78 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:500~2000, IF 1:100, FLOW 1:100
Predicted MW Explanation 19.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens peptide deformylase (mitochondrial) (PDF)
Alternative Name
Database LinkNP_071736
Entrez Gene 64146 Human
FunctionProtein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells. [provided by RefSeq].
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDF (RC205788, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDF.
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Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-PDF monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
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Anti-PDF mouse monoclonal antibody (TA502988) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDF(RC205788 ).
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HEK293T cells transfected with either RC205788 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDF antibody(TA502988), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-PDF antibody(TA502988),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-PDF antibody(TA502988),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
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