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Home Antibody All anti-PDE4A antibodies

Anti-PDE4A TRUEMAB Antibody clone 3D4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA501198
  • anti-PDE4A mouse monoclonal antibody, clone 3D4
100ul $325 In Stock
CF501198
  • Carrier-free (BSA/glycerol-free) PDE4A mouse monoclonal antibody, clone 3D4
100ug $450 3-4 weeks
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WB(1)
IHC(2)
IF(2)
FC(2)
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Also for PDE4A (NM_006202)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PDE4A (NP_006193) produced in HEK293T cell.
Clone Nameclone 3D4 IsotypeIgG1
Species ReactivityHuman Concentration0.64 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens phosphodiesterase 4A, cAMP-specific (PDE4A), transcript variant 4
Alternative NameDPDE2; PDE4; PDE46
Database LinkNP_006193
FunctionCyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. Cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. PDE4A is a class IV cAMP-specific PDE
Related Pathway
Signaling by GPCR

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4A (RC207765, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4A .
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-PDE4A mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501198, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-PDE4A mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501198, Dilution 1:50)
IF Image
Anti-PDE4A mouse monoclonal antibody (TA501198) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE4A (RC207765 ).
IF Image
Immunofluorescent staining of HT29 cells using anti-PDE4A mouse monoclonal antibody (TA501198).
FC Image
HEK293T cells transfected with either RC207765 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE4A antibody(TA501198), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-PDE4A antibody(TA501198),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

 

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