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Home Antibody All anti-PDE1B antibodies

Anti-PDE1B TRUEMAB Antibody Clone OTI6B9

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA503325 PDE1B mouse monoclonal antibody, clone OTI6B9 (formerly 6B9) 100ul $379 In Stock
LC400337 PDE1B HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503325 Carrier-free (BSA/glycerol-free) PDE1B mouse monoclonal antibody, clone OTI6B9 (formerly 6B9) 100ug $450 3-4 weeks
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WB(2)
IF(1)
FC(1)
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Also for PDE1B (NM_000924)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PDE1B(NP_000915) produced in HEK293T cell.
Clone NameClone OTI6B9 IsotypeIgG2b
Species ReactivityHuman, Dog, Rat, Monkey Concentration0.95 mg/ml
Guaranteed Application *WB, IF, FC Suggested DilutionsWB 1:500~2000, IF 1:100, FLOW 1:100,
Predicted MW Explanation 61.2 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 1
Alternative NamePDE1B1; PDES1B
Database LinkNP_000915
Entrez Gene 5153 Human
Entrez Gene 29691 Rat
Entrez Gene 705920 Monkey
Entrez Gene 100856025 Dog
FunctionCyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been classified into several families based on their biochemical properties. Members of the PDE1 family, such as PDE1B, are calmodulin (see MIM 114180)-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex (Repaske et al., 1992 [PubMed 1326532]).[supplied by OMIM].
Related PathwayDruggable Genome Purine metabolismCalcium signaling pathwayProgesterone-mediated oocyte maturation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE1B (RC206588, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE1B.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDE1B monoclonal antibody.
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Anti-PDE1B mouse monoclonal antibody (TA503325) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE1B(RC206588).
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HEK293T cells transfected with either RC206588 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE1B antibody(TA503325), and then analyzed by flow cytometry.
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