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Home Antibody All anti-PDE1B antibodies

Anti-PDE1B TRUEMAB Antibody Clone OTI6E4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA503319 PDE1B mouse monoclonal antibody, clone OTI6E4 (formerly 6E4) 100ul $325 In Stock
LC400337 PDE1B HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF503319 Carrier-free (BSA/glycerol-free) PDE1B mouse monoclonal antibody, clone OTI6E4 (formerly 6E4) 100ug $450 3-4 weeks
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WB(2)
IHC(1)
IF(1)
FC(2)
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Also for PDE1B (NM_000924)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human PDE1B(NP_000915) produced in HEK293T cell.
Clone NameClone OTI6E4 IsotypeIgG2b
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:150, IF 1:100, FLOW 1:100,
Predicted MW Explanation 61.2 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens phosphodiesterase 1B, calmodulin-dependent (PDE1B), transcript variant 1
Alternative NamePDE1B1; PDES1B
Database LinkNP_000915
Entrez Gene 5153 Human
FunctionCyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been classified into several families based on their biochemical properties. Members of the PDE1 family, such as PDE1B, are calmodulin (see MIM 114180)-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex (Repaske et al., 1992 [PubMed 1326532]).[supplied by OMIM].
Related PathwayDruggable Genome Purine metabolismCalcium signaling pathwayProgesterone-mediated oocyte maturation

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE1B (RC206588, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE1B.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDE1B monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PDE1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503319)
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Anti-PDE1B mouse monoclonal antibody (TA503319) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE1B(RC206588).
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HEK293T cells transfected with either RC206588 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PDE1B antibody(TA503319), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Jurkat cells, using anti-PDE1B antibody(TA503319),(Red), compared to a nonspecific negative control antibody,(Blue).
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