OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-PDCD4 antibodies

Anti-PDCD4 PHOSPHO Antibody 9G6

div

Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA319574 Mouse monoclonal Pdcd4 phospho S457 antibody (Phospho-specific) 100ug $325 3-7 Days
Add to Shopping Cart

spacer
WB(2)
IHC(1)

OriGene Data

ImmunogenThis monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding Ser457 of the human Pdcd4 protein.
Clone Name9G6 Isotype
Species Reactivityhuman, mouse, rat, xenopus Concentration1.0 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:20,000 - 1:100,000, WB: 1:2,000 - 1:10,000, IHC: 1:1,000 - 1:5,000
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Programmed cell death 4 (Pdcd4) is a novel tumor supressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphoryl-ation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.

Reference Data

Target NameHomo sapiens programmed cell death 4 (neoplastic transformation inhibitor) (PDCD4), transcript variant 2
Alternative NameH731
Database LinkNP_663314
Entrez Gene 27250 Human
Entrez Gene 18569 Mouse
Entrez Gene 64031 Rat
Function
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB using anti-Pdcd4 pS457 antibody shows detection of phosphorylated Pdcd4 (indicated by arrowhead at ~62 kDa) in NIH-3T3 cells after 5 min treatment with 30 ng/mL PDGF (lane 2). No reactivity is seen for unstimulated (non-phosphorylated) NIH 3T3 cells (lane 1). The membrane was probed with the primary antibody at a 1:2,000 dilution, overnight at 4?. For detection HRP conjugated Rb-a-Mouse IgG was used at a 1:20,000 dilution in blocking buffer for 1 h at 4?.
spacer
Western blot using Immunochemicals Protein A purified Mouse Monoclonal anti-Pdcd4 pS457 antibody against recombinant PDCD4 protein. Membrane was blocked in 1% BSA-TBS-T for 30 min RT and probed with 1° Ab Ms-A-Pdcd4pS457 1:1000 (o/n 4°C in 1% BSA-TBS-T) followed by 2° Ab Peroxidase Conjugated Rabbit anti Ms CUST10M Lot# 20121 at 1:40,000 in MB-070 30 min RT. Bands at ~62 kD and ~32 kD were detected.
spacer
Antibody TA319574 has been tested in IHC, analyzed by an anatomic pathologist and validated for use in IHC applications against formalin-fixed, paraffin-embedded human tissues. The antibody was serially diluted and tested at a range of concentrations on at least 22 different human formalin-fixed, paraffin archival tissues, and positive and negative tissues were scored and compared to the published literature on the expression and function of the gene.
spacer

 

spacer
Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title