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Anti-PCNA Antibody EPR3821

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307643
  • Rabbit monoclonal antibody against PCNA (clone EPR3821 )
  • FREE positive control: HEK293T cell transient overexpression lysate (LC400929) , 20ug
100ul $325 In Stock
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WB(1)
IHC(8)
IF(2)
FC(1)
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Also for PCNA (NM_002592)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human PCNA was used as an immunogen.
Clone NameEPR3821 IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:50; ICC: 1:100 - 1:250; IHC-P: 1:100 - 1:250; FC: 1:1000; ICC/IF: 1:200
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens proliferating cell nuclear antigen (PCNA), transcript variant 1
Alternative NameMGC8367
Database LinkNP_002583
FunctionProliferating cell nuclear antigen (PCNA) is a DNA polymerase accessory factor that is required for DNA replication during S phase of the cell cycle and for resynthesis during nucleotide excision repair of damaged DNA. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway (1). The ubiquitination of PCNA is an essential event in the operation of the DNA Damage Tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase (2).
Related Pathway
Cell cycle

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - PCNA antibody [EPR3821]; All lanes : Anti-PCNA antibody [EPR3821] at 1/50000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : 293T cell lysate.Lane 4 : A431 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 29 kDa.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PCNA antibody [EPR3821]; TA307643 at 1/100 dilution staining PCNA in Tonsil tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821]; TA307643 showing positive staining in Ovarian carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-PCNA antibody [EPR3821](TA307643); TA307643 showing negative staining in Normal brain tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-PCNA antibody [EPR3821](TA307643); TA307643 showing positive staining in Urinary bladder carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821]; TA307643 showing positive staining in Normal stomach tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821]; TA307643 showing positive staining in Normal colon tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821]; TA307643 showing positive staining in Breast carcinoma tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821]; TA307643 showing positive staining in Glioma tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [EPR3821]; TA307643 (1/200) staining PCNA in Hela cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to abreview.
IF Image
Immunocytochemistry/ Immunofluorescence - PCNA antibody [EPR3821]; TA307643 at 1/100 dilution staining PCNA in HeLa cells by Immunofluorescence.
FC Image
Flow Cytometry - Anti-PCNA antibody [EPR3821]; Overlay histogram showing HeLa cells stained with TA307643 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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