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Home Antibody All anti-PCNA antibodies

Anti-PCNA Antibody EPR3821

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA307643 Rabbit monoclonal antibody against PCNA (clone EPR3821 ) 100ul $325 In Stock
LC400929 PCNA HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(1)
IHC(8)
IF(2)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in human PCNA was used as an immunogen.
Clone NameEPR3821 IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB: 1:1000 - 1:10000; IP: 1:50; ICC: 1:100 - 1:250; IHC-P: 1:100 - 1:250; FC: 1:1000; ICC/IF: 1:200
Predicted MW Explanation 29 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens proliferating cell nuclear antigen (PCNA), transcript variant 1
Alternative NameATLD2
Database LinkNP_002583
Entrez Gene 5111 Human
Entrez Gene 18538 Mouse
Entrez Gene 25737 Rat
FunctionProliferating cell nuclear antigen (PCNA) is a DNA polymerase accessory factor that is required for DNA replication during S phase of the cell cycle and for resynthesis during nucleotide excision repair of damaged DNA. PCNA acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway (1). The ubiquitination of PCNA is an essential event in the operation of the DNA Damage Tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase (2).
Related PathwayStem cell - PluripotencyDruggable Genome DNA replicationBase excision repairNucleotide excision repairMismatch repairCell cycle

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - PCNA antibody [EPR3821]; All lanes : Anti-PCNA antibody [EPR3821] at 1/50000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : HepG2 cell lysate.Lane 3 : 293T cell lysate.Lane 4 : A431 cell lysate.Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 29 kDa.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PCNA antibody [EPR3821]; TA307643 at 1/100 dilution staining PCNA in Tonsil tissue by Immunohistochemistry Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821]; TA307643 showing positive staining in Ovarian carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-PCNA antibody [EPR3821](TA307643); TA307643 showing negative staining in Normal brain tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-PCNA antibody [EPR3821](TA307643); TA307643 showing positive staining in Urinary bladder carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821]; TA307643 showing positive staining in Normal stomach tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821]; TA307643 showing positive staining in Normal colon tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821]; TA307643 showing positive staining in Breast carcinoma tissue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCNA antibody [EPR3821]; TA307643 showing positive staining in Glioma tissue.
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Immunocytochemistry/ Immunofluorescence - Anti-PCNA antibody [EPR3821]; TA307643 (1/200) staining PCNA in Hela cells (green). Cells were fixed in methanol and counterstained with DAPI in order to highlight the nucleus (red).
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Immunocytochemistry/ Immunofluorescence - PCNA antibody [EPR3821]; TA307643 at 1/100 dilution staining PCNA in HeLa cells by Immunofluorescence.
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Flow Cytometry - Anti-PCNA antibody; Overlay histogram showing HeLa cells stained with TA307643 (red line). The secondary antibody used was Alexa Fluor 488 goat anti-rabbit IgG (H+L) at 1:2000. Isotype control antibody (black line) was rabbit IgG (monoclonal) used under the same conditions. Unlabelled sample (blue line) was also used as a control.
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