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Anti-PCK1 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for PCK1 (NM_002591)
|DNA immunization. This antibody is specific for the Middle Region of the target protein.|
|Human , Rat , Monkey , Mouse
|WB, ELISA, IHC
|| WB 1:5000~20000, IHC 1:150, ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens phosphoenolpyruvate carboxykinase 1 (soluble) (PCK1)|
|PEPCK-C; PEPCK1; PEPCKC|
Entrez Gene 5105 Human
Entrez Gene 18534 Mouse
Entrez Gene 362282 Rat
Entrez Gene 696207 Monkey
|Major sites of expression of PEP Carboxylase are liver, kidney and adipocytes. PEP carboxylase, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein has also been characterized.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PCK1 (RC204758, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PCK1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PCK1 polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western Blot: PCK1 Antibody - Samples: Lane 1, Marker: 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2, RT-4 Lane 3, U-251MG sp Lane 4, Human Plasma Lane 5, Liver Lane 6, Tonsil , Target weight: 69 Validation score: 2 Validation description: Supportive - Band of predicted size in kDa (+/-20%) with additional bands present.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-PCK1 Rabbit Polyclonal antibody. (TA590679)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-PCK1 Rabbit Polyclonal antibody. (TA590679)