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Anti-PCDH15 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for PCDH15 (NM_033056)
|DNA immunization. This antibody is specific for the N Terminus Region of the target protein (NM_023115).|
||WB: 1:5000-1:20000; ELISA: 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
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Western Blot: PCDH15 Antibody - Western Blot was performed using affinity purified SEQer PCDH15 antibody, aa(108-207) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer PCDH15 antibody, aa(108-207) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown.