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Anti-PARP1 Antibody 7A10
Also for PARP1 (NM_001618)
|A synthetic peptide corresponding to residues before the cleavage site of human PARP-1 was used as immunogen. The antibody only recognizes full-length form (p116) of PARP-1.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500-2000, IF: 1:200-400
|Supplied in 10 mM HEPES sodium (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol, and 0.03% sodium azide|
|N/A (Protein A or G Sepharose)
|Homo sapiens poly (ADP-ribose) polymerase 1 (PARP1)|
|ADPRT; ADPRT 1; ADPRT1; ARTD1; pADPRT-1; PARP; PARP-1; PPOL|
|PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress. This protein can be cleaved by many ICE-like Caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis.|
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Western blot analysis using PARP mouse mAb against Jurkat (1), K562 (2), HeLa (3), Raji (4), THP-1 (5) and SW620 (6) cell lysate.
Flow cytometric analysis of Jurkat cells using anti-PARP mAb (green) and negative control (purple).