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Anti-PANK2 TRUEMAB Antibody clone 3H9
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for PANK2 (NM_153638)
|Full length human recombinant protein of human PANK2(NP_705902) produced in HEK293T cell.|
|Human, Dog, Rat, Monkey
|WB, IHC, IF, FC
||WB 1:1000~2000, IHC 1:50, IF 1:100, FLOW 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens pantothenate kinase 2 (PANK2), transcript variant 1|
|C20orf48; HARP; HSS; NBIA1; PKAN|
|This gene encodes a protein belonging to the pantothenate kinase family and is the only member of that family to be expressed in mitochondria. Pantothenate kinase is a key regulatory enzyme in the biosynthesis of coenzyme A (CoA) in bacteria and mammalian cells. It catalyzes the first committed step in the universal biosynthetic pathway leading to CoA and is itself subject to regulation through feedback inhibition by acyl CoA species. Mutations in this gene are associated with HARP syndrome and pantothenate kinase-associated neurodegeneration (PKAN), formerly Hallervorden-Spatz syndrome. Alternative splicing, involving the use of alternate first exons, results in multiple transcripts encoding different isoforms. [provided by RefSeq].|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PANK2 (RC215676, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PANK2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PANK2 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-PANK2 mouse monoclonal antibody. (TA501321, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-PANK2 mouse monoclonal antibody. (TA501321, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-PANK2 mouse monoclonal antibody. (TA501321, Dilution 1:50)
Anti-PANK2 mouse monoclonal antibody (TA501321) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PANK2(RC215676).
HEK293T cells transfected with either RC215676 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PANK2 antibody(TA501321), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-PANK2 antibody(TA501321),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).