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Home Antibody All anti-PAK2 antibodies

Anti-PAK2 Antibody EP796Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303627
  • Rabbit monoclonal antibody against PAK 2 (EP796Y )
100ul 325 3-7 Days
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WB(1)
IHC(1)
FC(1)
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Also for PAK2 (NM_002577)
cDNA Clone shRNA/siRNA Primer Pair Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human PAK 2 was used as an immunogen.
Clone NameEP796Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB: 1:1000; IHC: 1:100; ICC: 1:100 - 250; FC: 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens p21 protein (Cdc42/Rac)-activated kinase 2 (PAK2)
Alternative NamePAK65; PAKgamma
Database LinkNP_002568
FunctionThe p21 activated kinases (PAK) are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. The PAK proteins are a family of serine/threonine kinases that serve as targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities. The protein encoded by this gene is activated by proteolytic cleavage during caspase-mediated apoptosis, and may play a role in regulating the apoptotic events in the dying cell. [provided by RefSeq].
Related Pathway
Focal Adhesion
MAPK signaling pathway

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WB Image
Western blot - PAK2 antibody [EP796Y]; Anti-PAK2 antibody [EP796Y] at 1/2000 dilution + HeLa cell lysate at 10 µg.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 58 kDa.Observed band size : 61 kDa .
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAK2 antibody [EP796Y]; TA303627, at a 1/100 dilution, staining PAK2 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
FC Image
Flow Cytometry - Anti-PAK2 antibody [EP796Y]; Overlay histogram showing HeLa cells stained with TA303627 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was Alexa Fluor? 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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