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Anti-PAK1 Antibody EP795Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA303624
  • Rabbit monoclonal antibody against PAK1 (N-term)(EP795Y)
100ul 325 3-7 Days
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WB(1)
IHC(1)
IF(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues in the N-terminus of human PAK1 protein was used as immunogen.
Clone NameEP795Y IsotypeIgG
Species ReactivityHuman, Mouse, Rat Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsFC: 1:10; IP: 1:50; WB: 1:500; IHC-P: Use at an assay dependent dilution; ICC/IF: 1:50 - 1:100
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens p21 protein (Cdc42/Rac)-activated kinase 1 (PAK1), transcript variant 2
Alternative NamePAKalpha
Database LinkNP_002567
FunctionPAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
Related Pathway
EGFR1 Signaling Pathway
Focal Adhesion
MAPK signaling pathway

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WB Image
Western blot - PAK1 antibody [EP795Y]; .Predicted band size : 66 kDa.Observed band size : 66 kDa.
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - PAK1 antibody [EP795Y]; Immunohistochemical analysis of paraffin-embedded human breast carcinoma using TA303624.
IF Image
Immunocytochemistry/ Immunofluorescence - PAK1 antibody [EP795Y]; Immunofluorescent staining of HeLa cells using TA303624.
FC Image
Flow Cytometry-Anti-PAK1 antibody [EP795Y](TA303624); Overlay histogram showing HeLa cells stained with TA303624 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

 

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