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Home Antibody All anti-OTUB1 antibodies

Anti-OTUB1 TRUEMAB Antibody Clone OTI5B8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA505150
  • OTUB1 mouse monoclonal antibody, clone OTI5B8 (formerly 5B8)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402607) , 20ug Explanation
100ul $379 In Stock
CF505150
  • Carrier-free (BSA/glycerol-free) OTUB1 mouse monoclonal antibody, clone OTI5B8 (formerly 5B8)
100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type

Buy any antibody of 100ul or more, get a free package of 3 loading control antibody samples. View Details Add to Shopping Cart

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WB(1)
IHC(10)
FC(1)
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Also for OTUB1 (NM_017670)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human OTUB1(NP_060140) produced in HEK293T cell.
Clone NameClone OTI5B8 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 31.1 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens OTU deubiquitinase, ubiquitin aldehyde binding 1 (OTUB1), transcript variant 1
Alternative NameHSPC263; OTB1; OTU1
Database LinkNP_060140
Entrez Gene 55611 Human
FunctionThe product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq].
Related PathwayProtease

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OTUB1 (RC210648, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OTUB1.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-OTUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505150)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-OTUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505150)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-OTUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505150)
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-OTUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505150)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-OTUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505150)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-OTUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505150)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-OTUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505150)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-OTUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505150)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-OTUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505150)
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-OTUB1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA505150)
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HEK293T cells transfected with either RC210648 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-OTUB1 antibody(TA505150), and then analyzed by flow cytometry.
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