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Anti-ORP1 Antibody EPR8646
Also for ORP1 (NM_018030)
|A synthetic peptide corresponding to residues on the C-terminus of human ORP1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; FC: 1:10 - 1:100
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC or IHC-P.
|Homo sapiens oxysterol binding protein-like 1A (OSBPL1A), transcript variant 1|
|ORP-1; ORP1; OSBPL1B|
|ORP1 is a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain (1). ORP1 binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate. Three ORP1 isoforms have been reported (isoform A: 50 kDa, isoform B: 108 kDa, isoform C: 106 kDa) (2).|
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Western blot - Anti-ORP1 antibody [EPR8646]; All lanes : Anti-ORP1 antibody [EPR8646] at 1/1000 dilution.Lane 1 : A549 cell lysate.Lane 2 : 293T cell lysate.Lane 3 : U87-MG cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled goat anti-rabbit IgG at 1/2000 dilution.Predicted band size : 108 kDa.Observed band size : 50-108 kDa .
Flow Cytometry - Anti-ORP1 antibody [EPR8646]; ab131165 at 1/10 dilution staining ORP1 in permeabilized A549 cells by Flow cytometry (red). Rabbit IgG negative control (green).