Home Antibody All anti-OLIG2 antibodies
Anti-OLIG2 GAT Antibody Polyclonal
Generated by Genomic Immunization
Also for OLIG2 (NM_005806)
|DNA immunization. This antibody was made against a protein fragment from the N Terminus Region|
|WB, IHC, IF
|| WB 1:20000, ELISA 1:100-1:2000
|20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.0|
|The fragment used for DNA immunization was expressed in E.coli and the purified protein fragment was used for affinity purification of the antibody.
|This antibody was generated by SDIX's Genomic Antibody Technology ® (GAT). Learn about GAT
|Homo sapiens oligodendrocyte lineage transcription factor 2 (OLIG2)|
|BHLHB1; bHLHe19; OLIGO2; PRKCBP2; RACK17|
|Olig2 (oligodendrocyte transcription factor 2) is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. It cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. It is an antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development.|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OLIG2 (RC208209, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OLIG2.
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in a subset of glial cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleoli & golgi apparatus.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.