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Anti-OBFC1 TRUEMAB Antibody clone 2E4
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for OBFC1 (NM_024928)
|Full length human recombinant protein of human OBFC1 (NP_079204) produced in HEK293T cell.|
|WB, IF, FC
||WB 1:2000, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens oligonucleotide/oligosaccharide-binding fold containing 1 (OBFC1)|
|AAF-44; AAF44; bA541N10.2; RPA-32; STN1|
Entrez Gene 79991 Human
|OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al., 2009 [PubMed 19854130]).[supplied by OMIM]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OBFC1 (RC200778, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OBFC1.
Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-OBFC1 monoclonal antibody at 1:200 dilution.
Anti-OBFC1 mouse monoclonal antibody (TA503240) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OBFC1(RC200778).
HEK293T cells transfected with either RC200778 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-OBFC1 antibody(TA503240), and then analyzed by flow cytometry.