OriGene Technologies, Inc.
Search:    
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
 
Home Antibody All anti-Nphs1 antibodies

Anti-Nphs1 PHOSPHO Antibody EPTPG1

div

Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307275
  • Rabbit monoclonal antibody against Nephrin Phospho (pY1176/1193) (clone EPR2883Y ) (Phospho-Specific)
100ul 379 3-7 Days
Add to Shopping Cart
WB(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues surrounding tyrosine 1176 and 1193 of mouse Nephrin was used an immunogen. This antibody detects both Nephrin phosphorylated on tyrosines 1176 and 1193 as well as non-phosphorylated Nephrin.
Clone NameEPTPG1 IsotypeIgG
Species ReactivityMouse, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:10000 - 1:50000
Predicted MW Explanation 138 kDa
BufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens nephrosis 1, congenital, Finnish type (nephrin) (NPHS1)
Alternative NameCNF; nephrin; NPHN
Database LinkNP_004637
Entrez Gene 4868 Human
Entrez Gene 54631 Mouse
FunctionNephrin (NPHS1) is an immunoglobulin family adhesion protein expressed in visceral glomerular epithelial cells (podocytes), where it is targeted to the intercellular slit diaphragm of mature foot processes (1). It is an essential component of the kidney filtration barrier, and mutations in the nephrin gene are associated with congenital nephrotic syndrome (2). Nephrin binds via phosphorylated tyrosine residues in the intracellular domain to SH2 domain-containing signaling molecules such as Nck (3).
Related PathwayTransmembraneDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1]; All lanes : Anti-Nephrin (phospho Y1176 + Y1193) antibody [EPTPG1] at 1/50000 dilution.Lane 1 : HEK293T cells transfected with wild type CD 16/7 Nephrin.Lane 2 : HEK293T cells transfected with active tyrosine kinase (vSrc).Lane 3 : HEK293T cells transfected with Nephrin mutated at Tyr1176 and vSrc.Lane 4 : HEK293T cells transfected with Nephrin mutated at Tyr 1193 and vSrc using Phospho Nephrin (pY1176/1193).Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 138 kDa.Observed band size : 138,180 kDa .

 

spacer
Inc 5000 Healthcare Company Copyright © 2014 OriGene Technologies, Inc. All Rights Reserved. Legal Notices.
9620 Medical Center Dr., Suite 200, Rockville, MD 20850 • 1.888.267.4436

Reproduction of any materials from this website is strictly forbidden without permission.

All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title