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Anti-NUMB TRUEMAB Antibody Clone OTI1A7
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for NUMB (NM_003744)
|Full length human recombinant protein of human NUMB (NP_003735) produced in HEK293T cell.|
|WB, IF, FC
||WB 1:2000, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens numb homolog (Drosophila) (NUMB), transcript variant 3|
|C14orf41; c14_5527; S171|
Entrez Gene 8650 Human
|The protein encoded by this gene plays a role in the determination of cell fates during development. The encoded protein, whose degradation is induced in a proteasome-dependent manner by MDM2, is a membrane-bound protein that has been shown to associate with EPS15, LNX1, and NOTCH1. Four transcript variants encoding different isoforms have been found for this gene.|
| Notch signaling pathway|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUMB (RC216230, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUMB.
Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-NUMB monoclonal antibody.(1:200)
Anti-NUMB mouse monoclonal antibody (TA501614) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NUMB(RC216230 ).
HEK293T cells transfected with either RC216230 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NUMB antibody(TA501614), and then analyzed by flow cytometry.