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Anti-NUF2 Antibody


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA307991 Rabbit polyclonal antibody to CDCA1 (CDCA1 , NDC80 kinetochore complex component, homolog (S. cerevisiae)) 100ul $325 3-7 Days
LC430140 NUF2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

Clone Name IsotypeIgG
Species ReactivityHuman Concentration0.35mg/ml
Guaranteed Application *WB, IF Suggested DilutionsICC/IF:1:100-1:1000; WB:1:1000-1:10000
Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Purification Purified by antigen-affinity chromatography.

Reference Data

Target NameHomo sapiens NUF2, NDC80 kinetochore complex component (NUF2), transcript variant 1
Alternative NameCDCA1; CT106; NUF2R
Database LinkNP_663735
Entrez Gene 83540 Human
FunctionThis gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described. [provided by RefSeq]
Related PathwayDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Sample (30 ug of whole cell lysate). A: HeLa. B: HeLa nucleus. 10% SDS PAGE. TA307991 diluted at 1:5000.

IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using CDCA1(TA307991) antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with anti-alpha tubulin antibody (Red) at 1:2000.


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