Anti-NUBPL Antibody, clone 1C5

Also for NUBPL (NM_025152)

OriGene Data

Immunogen	Human recombinant protein fragment corresponding to amino acids 1-250 of human NUBPL(NP_079428) produced in E.coli.
Clone Name	clone 1C5	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IHC, FC	Suggested Dilutions	WB 1:500~2000, IHC 1:150, FLOW 1:100,
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens nucleotide binding protein-like (NUBPL), nuclear gene encoding mitochondrial protein, transcript variant 1
Alternative Name	C14orf127; huInd1; IND1
Database Link	NP_079428
Function	This gene encodes a member of the Mrp/NBP35 ATP-binding proteins family. The encoded protein is required for the assembly of the respiratory chain NADH dehydrogenase (complex I), an oligomeric enzymatic complex located in the inner mitochondrial membrane. The respiratory complex I consists of 45 subunits and 8 iron-sulfur (Fe/S) clusters. This protein is an Fe/S protein that plays a critical role in the assembly of respiratory complex I, likely by transferring Fe/S into the Fe/S-containing complex I subunits. Mutations in this gene cause mitochondrial complex I deficiency. Alternatively spliced transcript variants encoding distinct isoforms have been identified.
Related Pathway

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WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NUBPL (RC204385, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NUBPL.

IHC Image

Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-NUBPL mouse monoclonal antibody. (TA503726)

IHC Image

Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-NUBPL mouse monoclonal antibody. (TA503726)

IHC Image

Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-NUBPL mouse monoclonal antibody. (TA503726)

IHC Image

Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-NUBPL mouse monoclonal antibody. (TA503726)

FC Image

HEK293T cells transfected with either RC204385 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NUBPL antibody(TA503726), and then analyzed by flow cytometry.

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Anti-NUBPL TrueMAB Antibody clone 1C5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data