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Anti-NTRK1 Antibody EP1056Y
Also for NTRK1 (NM_002529)
|A synthetic peptide corresponding to residues surrounding tyrosine 680 of human Trk was used as an immunogen. This antibody detects both phosphorylated and unphosphorylated Trk.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:10000 - 1:20000; IP: 1:20; FC: 1:60
|Preservative: 0.01% Sodium azide|
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for ICC or IHC-P.
|Homo sapiens neurotrophic tyrosine kinase, receptor, type 1 (NTRK1), transcript variant 2|
|MTC; p140-TrkA; TRK; Trk-A; TRK1; TRKA|
|The trk proto-oncogene encodes a 140-kilodalton, membrane-spanning protein Trk that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of Trk in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments indicate that Trk binds NGF specifically in cultured cells with a dissociation constant of 10(-9) molar. The identification of Trk as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF (1). Nerve growth factor and its high-affinity receptor Trk are thought to be involved in the progression of various cancers (2). NGF interacts with two different low-affinity receptors that can be distinguished by affinity crosslinking. Reconstitution experiments by membrane fusion and transient transfection into heterologous cells indicate that high-affinity NGF binding requires coexpression and binding to both the low-affinity NGF receptor and Trk. These studies reveal a new growth factor receptor-mediated mechanism of cellular differentiation involving Trk and the low-affinity NGF receptor (3). |
MAPK signaling pathway
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Western blot - Anti-TrkA antibody [EP1056Y]; Anti-TrkA antibody [EP1056Y] at 1/20000 dilution + Human brain tissue lysate at 10 µg.Secondary.Goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 87 kDa.