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Home Antibody All anti-NRBP1 antibodies

Anti-NRBP1 TRUEMAB Antibody Clone OTI4C1

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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SKU Description Amount Price Availability*  
TA500461 anti-NRBP1 (MADM) mouse monoclonal antibody, clone OTI4C1 (formerly 4C1) 100ul $325 In Stock
LC415614 NRBP1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF500461 Carrier-free (BSA/glycerol-free) NRBP1 mouse monoclonal antibody, clone OTI4C1 (formerly 4C1) 100ug $450 3-4 weeks
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WB(2)
IP(1)
IHC(3)
FC(1)
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Also for NRBP1 (NM_013392)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenFull-length protein expressed in 293T cell transfected with human NRBP1 expression vector
Clone NameClone OTI4C1 IsotypeIgG2b
Species ReactivityHuman Concentration1.3 mg/ml
Guaranteed Application *WB, IHC, IP, FC Suggested DilutionsWB 1:1000~2000, IHC 1:50, FLOW 1:100, IP 2ug/500ul
Predicted MW Explanation 59.8 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens nuclear receptor binding protein 1 (NRBP1)
Alternative NameBCON3; MADM; MUDPNP; NRBP
Database LinkNP_037524
Entrez Gene 29959 Human
FunctionMay play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication
Related PathwayProtein KinaseDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRBP1 (RC200107, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRBP1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NRBP1 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NRBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500461)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NRBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500461)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NRBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500461)
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Immunoprecipitation(IP) of NRBP1 by using TrueMab monoclonal anti-NRBP1 antibodies (Negative control: IP without adding anti-NRBP1 antibody.). For each experiment, 500ul of DDK tagged NRBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NRBP1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
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HEK293T cells transfected with either RC200107 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NRBP1 antibody(TA500461), and then analyzed by flow cytometry.
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