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Anti-NRBP1 TRUEMAB Antibody Clone OTI6C11
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for NRBP1 (NM_013392)
|Full-length protein expressed in 293T cell transfected with human NRBP1 expression vector|
|WB, IHC, IP, FC
||WB 1:200~500, IHC 1:50, FLOW 1:100, IP 2ug/500ul
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NRBP1 (RC200107, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NRBP1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NRBP1 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-NRBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500443)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-NRBP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500443)
Immunoprecipitation(IP) of NRBP1 by using TrueMab monoclonal anti-NRBP1 antibodies (Negative control: IP without adding anti-NRBP1 antibody.). For each experiment, 500ul of DDK tagged NRBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-NRBP1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC200107 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NRBP1 antibody(TA500443), and then analyzed by flow cytometry.