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Home Antibody All anti-NRAS antibodies

Anti-NRAS Antibody EP1125Y

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA300732 Rabbit Monoclonal Antibody against NRAS (Clone EP1125Y) 100ul $325 In Stock
LC400901 NRAS HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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WB(2)
FC(1)

OriGene Data

Immunogen A synthetic peptide corresponding to residues near the N-terminal region of human Ras was used as an immunogen. Predicted to react with H-Ras and K-Ras.
Clone NameEP1125Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:500,000; FC: 1: 100; IP: 1:30
Predicted MW Explanation 18 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS)
Alternative NameALPS4; CMNS; N-ras; NCMS; NRAS1; NS6
Database LinkNP_002515
Entrez Gene 4893 Human
Entrez Gene 18176 Mouse
Entrez Gene 24605 Rat
FunctionRas (p21), a signal transducer, was first characterized as the transforming genes of Harvey & Kristen sarcoma virus (1). The Ras family (H-Ras, N-Ras, and K-Ras) regulates cell growth, differentiation and apoptosis (2). Switching from an active or resting state, Ras can either bind GTP or GDP respectively. In the triphosphate conformation, Ras will interact with GTPase activating protein (GAP) to increase its activity (3). Mutations in any of the three isoforms can convert these proteins into active oncogenes. Additionally, Ras mutations are found in 30 % of all human cancer (4).
Related PathwayDruggable Genome MAPK signaling pathwayErbB signaling pathwayChemokine signaling pathwayAxon guidanceVEGF signaling pathwayTight junctionMore Pathways >>

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Ras antibody [EP1125Y]; Anti-Ras antibody [EP1125Y] at 1/500000 dilution + C6 cell lysate at 10 ug/ml.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 18 kDa.Observed band size : 18 kDa.
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Western blot ; Anti-Ras antibody [EP1125Y] at 1/500 dilution + Human Ras full length protein at 0.01 ug.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Exposure time : 1 minute
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Flow Cytometry - Ras antibody [EP1125Y]; TA300732 at 1/100 dilution staining Ras in permeabilized PC-12 cells by flow cytometry (red). Rabbit IgG negative control (green).
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