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Home Antibody All anti-NRAS antibodies

Anti-NRAS Antibody EP1125Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300732
  • Rabbit Monoclonal Antibody against NRAS (Clone EP1125Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400901) , 20ug Explanation
100ul 325 In Stock
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WB(2)
FC(1)
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Also for NRAS (NM_002524)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

Immunogen A synthetic peptide corresponding to residues near the N-terminal region of human Ras was used as an immunogen. Predicted to react with H-Ras and K-Ras.
Clone NameEP1125Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, FC Suggested DilutionsWB: 1:500,000; FC: 1: 100; IP: 1:30
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens neuroblastoma RAS viral (v-ras) oncogene homolog (NRAS)
Alternative NameALPS4; CMNS; N-ras; NCMS; NRAS1; NS6
Database LinkNP_002515
Entrez Gene 4893 Human
Entrez Gene 18176 Mouse
Entrez Gene 24605 Rat
FunctionRas (p21), a signal transducer, was first characterized as the transforming genes of Harvey & Kristen sarcoma virus (1). The Ras family (H-Ras, N-Ras, and K-Ras) regulates cell growth, differentiation and apoptosis (2). Switching from an active or resting state, Ras can either bind GTP or GDP respectively. In the triphosphate conformation, Ras will interact with GTPase activating protein (GAP) to increase its activity (3). Mutations in any of the three isoforms can convert these proteins into active oncogenes. Additionally, Ras mutations are found in 30 % of all human cancer (4).
Related Pathway
EGFR1 Signaling Pathway
Hemostasis
MAPK signaling pathway
Melanogenesis

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - Ras antibody [EP1125Y]; Anti-Ras antibody [EP1125Y] at 1/500000 dilution + C6 cell lysate at 10 ug/ml.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 18 kDa.Observed band size : 18 kDa.
WB Image
Western blot ; Anti-Ras antibody [EP1125Y] at 1/500 dilution + Human Ras full length protein at 0.01 ug.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Exposure time : 1 minute
FC Image
Flow Cytometry - Ras antibody [EP1125Y]; TA300732 at 1/100 dilution staining Ras in permeabilized PC-12 cells by flow cytometry (red). Rabbit IgG negative control (green).

 

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