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Anti-NRAS Antibody EP1125Y
Also for NRAS (NM_002524)
| A synthetic peptide corresponding to residues near the N-terminal region of human Ras was used as an immunogen. Predicted to react with H-Ras and K-Ras.|
|Mouse, Rat, Human
||Lot dependent; please refer to CoA along with shipment
||WB: 1:500,000; FC: 1: 100; IP: 1:30
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant
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Western blot - Ras antibody [EP1125Y]; Anti-Ras antibody [EP1125Y] at 1/500000 dilution + C6 cell lysate at 10 ug/ml.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Predicted band size : 18 kDa.Observed band size : 18 kDa.
Western blot ; Anti-Ras antibody [EP1125Y] at 1/500 dilution + Human Ras full length protein at 0.01 ug.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Exposure time : 1 minute
Flow Cytometry - Ras antibody [EP1125Y]; TA300732 at 1/100 dilution staining Ras in permeabilized PC-12 cells by flow cytometry (red). Rabbit IgG negative control (green).