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Anti-NPM1 Phospho Antibody EP1857Y
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Also for NPM1 (NM_001037738)
|A phospho-specific peptide corresponding to residues surrounding Theonine 199 was used as an immunogen. This antibody recognizes proteins phosphorylated at T199.|
|WB, IHC, IF
||WB: 1:25,00 10,000; IHC: 1:100 250; ICC: 1:100 250; IP: 1:70
|50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.|
|Homo sapiens nucleophosmin (nucleolar phosphoprotein B23, numatrin) (NPM1), transcript variant 3|
|NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF (MIM 600160). NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia (AML; MIM 601626) (Garzon et al., 2008 [PubMed 18308931]).[supplied by OMIM]. |
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Western blot analysis on HeLa cell lysates using anti-Phospho-NPM (pT199) RabMAb (TA303621), 1:50,000 dilution. Cells were either (A) untreated (B) treated with CA.
Immunohistochemical analysis of paraffin-embedded human lymphoma using anti-NPM (pT199) RabMAb (TA303621).
Immunofluorescent staing of HeLa cells using anti-Phospho-NPM (pT199) RabMAb (TA303621).