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Anti-NPM1 PHOSPHO Antibody EP1857Y
Also for NPM1 (NM_199185)
|A phospho-specific peptide corresponding to residues surrounding Theonine 199 was used as an immunogen. This antibody recognizes proteins phosphorylated at T199.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:2500 - 1:10000; IHC-P: 1:100 - 1:250; ICC/IF: 1:100 - 1:250; IP: 1:70; FC: 1:100
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Tissue culture supernatant (Protein A or G Sepharose)
|Does not react with Mouse, Rat
|Homo sapiens nucleophosmin (nucleolar phosphoprotein B23, numatrin) (NPM1), transcript variant 2|
|NPM1 is a ubiquitously expressed nucleolar protein that shuttles between the nucleus and cytoplasm. It is implicated in multiple functions, including ribosomal protein assembly and transport, control of centrosome duplication, and regulation of the tumor suppressor ARF (MIM 600160). NPM1 mutations that relocalize NPM1 from the nucleus into the cytoplasm are associated with development of acute myeloid leukemia (AML; MIM 601626) (Garzon et al., 2008 [PubMed 18308931]).[supplied by OMIM]. |
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Western blot - Nucleophosmin (phospho Y199) antibody [EP1857Y]; All lanes : Anti-Nucleophosmin (phospho T199) antibody [EP1857Y] at 1/2500 dilution.Lane 1 : lysate from untreated HeLa cells .Lane 2 : lysate from HeLa cells treated with CA.Lysates/proteins at 10 µg per lane.Secondary.HRP labelled Goat anti-Rabbit at 1/2000 dilution.Predicted band size : 33 kDa.Observed band size : 33 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nucleophosmin (phospho Y199) antibody [EP1857Y]; Immunohistochemistry analysis of paraffin-embedded Human lymphoma tissue, using 1/100 ab81551.
Immunocytochemistry/ Immunofluorescence - Nucleophosmin (phospho Y199) antibody [EP1857Y]; Immunofluorescent staing of HeLa cells using 1/100 ab81551.
Immunocytochemistry/ Immunofluorescence - Nucleophosmin (phospho T199) antibody [EP1857Y]; ab81551 stainingNucleophosmin (phospho T199) inhuman HeLa cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with formaldehyde, permeabilized with 0.1%Triton x100in PBS and blocking with 5% serum was performed for 30 minutes at 230C. Samples were incubated with primary antibody (1/150 in PBS) for 1 hour at 23°C. An Alexa Fluor?488-conjugatedgoat polyclonal torabbit IgG was used at dilution at 1/100 as secondary antibody.
Flow Cytometry-Anti-Nucleophosmin (phospho T199) antibody [EP1857Y](ab81551); Overlay histogram showing HeLa cells stained with (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody , 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.