OriGene Technologies, Inc.
Left ProductsProducts divider ServicesServices divider technologyTechnology divider researchResearch divider TechsupportTechSupport divider AboutAbout Right
Home Antibody All anti-Nphs1 antibodies

Anti-Nphs1 PHOSPHO Antibody EPTPG3


Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
  • Rabbit monoclonal antibody against Nephrin Phospho (pY1217) (clone EPTPG1 ) (Phospho-Specific)
100ul $379 3-7 Days
Add to Shopping Cart

OriGene Data

ImmunogenA synthetic peptide corresponding to residues surrounding tyrosine 1217 of mouse Nephrin was used an immunogen. This antibody detects both Nephrin phosphorylated on tyrosine 1217.
Clone NameEPTPG3 IsotypeIgG
Species ReactivityMouse, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsWB: 1:10000 - 1:50000; IP: 1:20000
Predicted MW Explanation 138 kDa
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant

Reference Data

Target NameHomo sapiens nephrosis 1, congenital, Finnish type (nephrin) (NPHS1)
Alternative NameCNF; nephrin; NPHN
Database LinkNP_004637
Entrez Gene 4868 Human
Entrez Gene 54631 Mouse
FunctionNephrin (NPHS1) is an immunoglobulin family adhesion protein expressed in visceral glomerular epithelial cells (podocytes), where it is targeted to the intercellular slit diaphragm of mature foot processes (1). It is an essential component of the kidney filtration barrier, and mutations in the nephrin gene are associated with congenital nephrotic syndrome (2). Nephrin binds via phosphorylated tyrosine residues in the intracellular domain to SH2 domain-containing signaling molecules such as Nck (3).
Related PathwayTransmembraneDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Nephrin (phospho Y1217) antibody [EPTPG3]; All lanes : Anti-Nephrin (phospho Y1217) antibody [EPTPG3] at 1/50000 dilution.Lane 1 : HEK293T cells transfected with wild type CD 16/7 Nephrin.Lane 2 : HEK293T cells transfected with active tyrosine kinase (vSrc).Lane 3 : HEK293T cells transfected with Nephrin mutated at Tyr1217 and vSrc using Phospho Nephrin (pY1176/1193).Lysates/proteins at 10 ug per lane.Secondary.HRP labelled goat anti-rabbit at 1/2000 dilution.Predicted band size : 138 kDa.Observed band size : 138 kDa.


Inc 5000 Healthcare Company
All Products by: Title | Price | Category | Popularity | Best Sellers Topselling Products by: Title | Price | Category | Popularity | Favorites
Popular Categories: Popularity | Our Choices | All-Round Favorites | Title Topselling Categories: Popularity | Our Choices | All-Round Favorites | Title