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Home Antibody All anti-NOTCH2 antibodies

Anti-NOTCH2 Antibody

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SKU Description Amount Price Availability*  
TA319403 Rabbit polyclonal anti-NOTCH 2 antibody 200ul $325 3-7 Days
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WB(1)
IHC(1)

OriGene Data

ImmunogenThis whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to amino acid residues 2396-2409 of human Notch 2 (the total protein is 2471 aa).  A residue of cysteine was added to the amino terminal end to facilitate coupling.
Clone Name IsotypeIgG
Species Reactivityhuman, dog, chimpanzee Concentration75 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:30,000 - 1:90,000, WB: 1:400 - 1:2,000, IHC: 1:200-1:800
Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Note Anti Notch 2 Antibody recognizes Notch 2 that is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.  Notch functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Reference Data

Target NameHomo sapiens notch 2 (NOTCH2), transcript variant 1
Alternative NameAGS2; HJCYS; hN2
Database LinkNP_077719
Entrez Gene 4853 Human
Entrez Gene 483148 Dog
Function
Related PathwayTransmembraneDruggable Genome Dorso-ventral axis formationNotch signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB using anti-Notch 2 (intra) antibody shows detection of a band at ~110 kDa corresponding to active Notch 2 protein (arrowhead). WB analysis was performed for Notch 2 expression using human mesothelial SV40 cells lysate obtained fromtransfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected). Anti-Notch 2 was used at 1:400.
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Anti-Notch 2 antibody was diluted 1:500 to detect NOTCH 2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
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