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Anti-NOTCH1 Antibody EP1238Y
Also for NOTCH1 (NM_017617)
|A synthetic peptide corresponding to the Cytoplasmic portion of human Notch1 was used as immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF
||IHC-P: 1:50 - 1:100; FC: 1:10 - 1:100; WB: 1:1000 - 1:10000; ICC/IF: 1:100 - 1:250
|Does not react with Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05% (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens notch 1 (NOTCH1)|
|Notch (hN1, Motch in mouse, Xotch in Xenopus) is a family of large transmembrane receptors involved in cell to cell interactions and cell fate decisions during invertebrate and vertebrate development. A modification of Notch signaling pathway can lead to changes in cellular proliferation, differentiation, and apoptotic events (1). Notch is activated by binding to membrane-bound ligands of the Delta and Serrate/Jagged family. Activated Notch receptor is proteolytically cleaved and the Notch intracellular domain (NICD) is released. The NICD binds to CSL DNA-binding protein and the Mastermind/Lag-3 co-activator in the nucleus which activates target gene expression (2,3). Notch has been implicated in T-cell acute lymphoblastic leukemia (T-All) and Multiple Sclerosis (MS). Also, repression of Notch signaling is commonly seen in cancer (4). In mammals, four different Notch receptors has been identified (Notch 1, 2, 3, and 4). Active Notch1 has been linked to inactivation of p53-dependent transactivation by inhibiting the phosphorylation of p53 protein (5). |
Delta-Notch Signaling Pathway
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Western blot - Notch1 antibody [EP1238Y]; Anti-Notch1 [EP1238Y] antibody at 1/500 dilution + Hek293 cell lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Observed band size : 125 kDa .
Immunohistochemistry (Paraffin-embedded sections) - Notch1 antibody [EP1238Y]; ab52627, at a 1/50 dilution, staining human Notch1 in fetal lung tissue, using Immunohistochemistry, Paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [EP1238Y]; ICC/IF image of ab52627 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.