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Home Antibody All anti-NOTCH1 antibodies

Anti-NOTCH1 Antibody EP1238Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300836
  • Rabbit Monoclonal Antibody against NOTCH1 (Clone EP1238Y)
  • FREE positive control: HEK293T cell transient overexpression lysate (LC402602) , 20ug
100ul 325 In Stock
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WB(1)
IHC(1)
IF(1)
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Also for NOTCH1 (NM_017617)
cDNA Clone shRNA/siRNA Lysate Protein Request Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to the Cytoplasmic portion of human Notch1 was used as immunogen.
Clone NameEP1238Y IsotypeIgG
Species ReactivityHuman,Mouse Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF Suggested DilutionsIHC-P: 1:50 - 1:100; FC: 1:10 - 1:100; WB: 1:1000 - 1:10000; ICC/IF: 1:100 - 1:250
BufferDoes not react with Rat. Is unsuitable for IP.
Purification PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens notch 1 (NOTCH1)
Alternative NamehN1; TAN1
Database LinkNP_060087
FunctionNotch (hN1, Motch in mouse, Xotch in Xenopus) is a family of large transmembrane receptors involved in cell to cell interactions and cell fate decisions during invertebrate and vertebrate development. A modification of Notch signaling pathway can lead to changes in cellular proliferation, differentiation, and apoptotic events (1). Notch is activated by binding to membrane-bound ligands of the Delta and Serrate/Jagged family. Activated Notch receptor is proteolytically cleaved and the Notch intracellular domain (NICD) is released. The NICD binds to CSL DNA-binding protein and the Mastermind/Lag-3 co-activator in the nucleus which activates target gene expression (2,3). Notch has been implicated in T-cell acute lymphoblastic leukemia (T-All) and Multiple Sclerosis (MS). Also, repression of Notch signaling is commonly seen in cancer (4). In mammals, four different Notch receptors has been identified (Notch 1, 2, 3, and 4). Active Notch1 has been linked to inactivation of p53-dependent transactivation by inhibiting the phosphorylation of p53 protein (5).
Related Pathway
Delta-Notch Signaling Pathway

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WB Image
Western blot - Notch1 antibody [EP1238Y]; Anti-Notch1 [EP1238Y] antibody at 1/500 dilution + Hek293 cell lysate at 10 µg.Secondary.goat anti-rabbit HRP at 1/2000 dilution.Observed band size : 125 kDa .
IHC Image
Immunohistochemistry (Paraffin-embedded sections) - Notch1 antibody [EP1238Y]; TA300836, at a 1/50 dilution, staining human Notch1 in fetal lung tissue, using Immunohistochemistry, Paraffin embedded tissue.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-Notch1 antibody [EP1238Y]; ICC/IF image of TA300836 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

 

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