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Anti-NONO Antibody EPR5269
Also for NONO (NM_001145408)
|A synthetic peptide corresponding to residues in human p54nrb was used as an immunogen.|
||Tissue culture supernatant
||Lot dependent; please refer to CoA along with shipment
||WB: 1:10000 - 1:50000; IHC-P: 1:50 - 1:100; ICC: 1:50 - 1:100; FC: 1:10 - 1:100
|Does not react with Rat. Is unsuitable for IP.|
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
|Is unsuitable for IP.
|Homo sapiens non-POU domain containing, octamer-binding (NONO), transcript variant 1|
|NMT55; NRB54; P54; P54NRB; PPP1R114|
|p54nrb (NONO) is a DNA- and RNA-binding protein which plays various roles in the nucleus, including transcriptional regulation and RNA splicing (1). As part of the splicesomal complex, PTB-associated RNA splicing factor (PSF) and p54nrb are essential for both step I and II pre-mRNA splicing reactions (2). p54nrb is a component of the SFPQ-NONO complex, which is associated with MATR3 and plays a role in nuclear retention of defective RNA’s. The complex is involved in DNA unwinding by modulating the function of topoisomerase I/TOP1 (3). A rearrangement between p54nrb and the transcription factor E3 has been observed in papillary renal cell carcinoma (1).|
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Western blot - nmt55 / p54nrb antibody [EPR5269]; All lanes : Anti-nmt55 / p54nrb antibody [EPR5269] at 1/10000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : 293T cell lysate.Lane 3 : Molt-4 cell lysate.Lane 4 : PC-3 cell lysate.Lysates/proteins at 10 µg per lane.Predicted band size : 54 kDa.Observed band size : 54 kDa.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - nmt55 / p54nrb antibody [EPR5269]; Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using TA311009 at a dilution of 1/50.