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Home Antibody All anti-NODAL antibodies

Anti-NODAL Antibody EP2058Y


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA301024 Rabbit Monoclonal Antibody against NODAL (Clone EP2058Y) 100ul $325 In Stock
LC402640 NODAL HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
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OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human Nodal was used as an immunogen. This antibody detects Pro-Nodal and Nodal.
Clone NameEP2058Y IsotypeIgG
Species ReactivityMouse, Human (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB Suggested DilutionsFC: 1:60; WB: 1:2000
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Is unsuitable for IHC-P or IP.

Reference Data

Target NameHomo sapiens nodal growth differentiation factor (NODAL)
Alternative NameHTX5
Database LinkNP_060525
Entrez Gene 4838 Human
Entrez Gene 18119 Mouse
FunctionVertebrates have characteristic and conserved left-right (L-R) visceral asymmetries, for example the left-sided heart. In humans, alterations of L-R development can have serious clinical implications, including cardiac defects. Results support a key role for nodal and nodal-related genes in interpreting and relaying L-R patterning information in vertebrates (1). Left-sided expression of Nodal in the lateral plate mesoderm is a conserved feature necessary for the establishment of normal left-right asymmetry during vertebrate embryogenesis. By using gain- and loss-of-function experiments in zebrafish and mouse, it has been shown that the activity of the Notch pathway is necessary and sufficient for Nodal expression around the node, and for proper left-right determination (2). During gastrulation, the three germ layers of the embryo are formed and organized along the anterior-posterior body axis. In the mouse, gastrulation involves the delamination of ectodermal cells through the primitive streak and their differentiation into mesoderm. The nodal signaling molecule is essential for mesoderm formation and subsequent organization of axial structures in early mouse development (3).
Related PathwayStem cell relevant signaling - TGFb/BMP signaling pathwaySecreted ProteinES Cell Differentiation/IPSCancer stem cellsEmbryonic stem cellsInduced pluripotent stem cellsDruggable Genome TGF-beta signaling pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Nodal antibody [EP2058Y]; Anti-Nodal antibody [EP2058Y] at 1/2000 dilution + Recombinant nodal protein at 10ng.Secondary.HRP conjugated goat anti-rabbit at 1/2000 dilution.Predicted band size : 39 kDa.Observed band size : 15 kDa .


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