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Home Antibody All anti-NNMT antibodies

Anti-NNMT TRUEMAB Antibody clone 3E3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA502521
  • NNMT mouse monoclonal antibody, clone 3E3
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401860) , 20ug Explanation
100ul $325 In Stock
CF502521
  • Carrier-free (BSA/glycerol-free) NNMT mouse monoclonal antibody, clone 3E3
100ug $450 3-4 weeks
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WB(1)
IHC(2)
FC(3)

OriGene Data

ImmunogenFull length human recombinant protein of human NNMT (NP_006160) produced in HEK293T cell.
Clone Nameclone 3E3 IsotypeIgG1
Species ReactivityHuman Concentration0.49 mg/ml
Guaranteed Application *WB, IHC, FC Suggested DilutionsWB 1:2000, IHC 1:150, FLOW 1:100
Predicted MW Explanation 29.4 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens nicotinamide N-methyltransferase (NNMT)
Alternative Namenicotinamide N-methyltransferase
Database LinkNP_006160
Entrez Gene 4837 Human
FunctionN-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq].
Related Pathway Nicotinate and nicotinamide metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NNMT (RC200641, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NNMT.
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502521)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NNMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502521)
FC Image
HEK293T cells transfected with either RC200641 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NNMT antibody(TA502521), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Hela cells, using anti-NNMT antibody(TA502521),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
FC Image
Flow cytometric Analysis of Jurkat cells, using anti-NNMT antibody(TA502521),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

 

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