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UltraMAB

NME1 UltraMAB Antibody, Clone UMAB94

UltraMAB™ Antibodies - Validated with 10K Protein Chip

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Specifications Citations (0) Related Products Product Documents
SKU Description Datasheet Price Availability*  
UM800025
  • NME1 mouse monoclonal antibody,clone UMAB94, 100 ul
$468 In Stock Add to Shopping Cart
UM870025
  • NME1 mouse monoclonal antibody,clone UMAB94, 30 ul
$120 In Stock Add to Shopping Cart
WB(2)
Data Img
IHC(8)
Data Img
IF(1)
Data Img
10K-CHIP(1)
Data Img

OriGene Data

ImmunogenFull length human recombinant protein of human NME1 (NP_937818) produced in E.coli.
Clone NameClone UMAB94 IsotypeIgG1
Species ReactivityHuman, Rat, Monkey, Dog Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB, IHC, IF, 10K-CHIP Suggested DilutionsIHC 1:100, IF 1:100
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens NME/NM23 nucleoside diphosphate kinase 1 (NME1), transcript variant 1
Alternative NameAWD; GAAD; NB; NBS; NDKA; NDPK-A; NDPKA; NM23; NM23-H1
Database LinkNP_937818
FunctionThis gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008].
Related Pathway

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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NME1 monoclonal antibody (Clone UMAB94).
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Western blot of human tissue lysates (15ug) from 10 different tissues (1: Testis, 2: Omentum, 3: Uterus, 4: Breast, 5: Brain, 6: Liver, 7: Ovary, 8: Thyroid, 9: Colon, 10: Spleen). Diluation: 1:500.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NME1 mouse monoclonal antibody. (UM800025; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NME1 mouse monoclonal antibody. (UM800025; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NME1 mouse monoclonal antibody. (UM800025; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NME1 mouse monoclonal antibody. (UM800025; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue using anti-NME1 mouse monoclonal antibody. (UM800025; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NME1 mouse monoclonal antibody. (UM800025; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NME1 mouse monoclonal antibody. (UM800025; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NME1 mouse monoclonal antibody. (UM800025; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Immunofluorescent staining of A549 cells using NME1 mouse monoclonal antibody (UM800025, green). Actin filaments were labeled with TRITC-phalloidin (red), and nuclear with DAPI (blue). The three-color overlay image is located at the bottom-right corner.
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OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-NME1 mouse monoclonal antibody (UM800025). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.
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