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Home Antibody All anti-NME1 antibodies

Anti-NME1 TRUEMAB Antibody Clone OTI25D8

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA801826 NME1 mouse monoclonal antibody, clone OTI25D8 (formerly 25D8) 100ul $325 In Stock
LC404982 NME1 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF801826 Carrier-free (BSA/glycerol-free) NME1 mouse monoclonal antibody, clone OTI25D8 (formerly 25D8) 100ug $450 3 Days
Conjugation is available for this antibody: choose conjugation type
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WB(1)
IHC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human NME1 (NP_937818) produced in E.coli.
Clone NameClone OTI25D8 IsotypeIgG1
Species ReactivityHuman Concentration1 mg/ml
Guaranteed Application *WB, IHC Suggested Dilutions WB 1:2000, IHC 1:150,
Predicted MW Explanation 19.5 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens NME/NM23 nucleoside diphosphate kinase 1 (NME1), transcript variant 1
Alternative NameAWD; GAAD; NB; NBS; NDKA; NDPK-A; NDPKA; NM23; NM23-H1
Database LinkNP_937818
Entrez Gene 4830 Human
FunctionThis gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2), which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq, Jul 2008].
Related PathwayStem cell - PluripotencyDruggable Genome Purine metabolismPyrimidine metabolismMetabolic pathways

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NME1 (RC220517, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NME1.
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Immunohistochemical staining of paraffin-embedded Human melanoma tissue using anti-NME1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA801826)
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