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Home Antibody All anti-NLN antibodies

Anti-NLN TRUEMAB Antibody Clone OTI3H4

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA504327 NLN mouse monoclonal antibody, clone OTI3H4 (formerly 3H4) 100ul $379 In Stock
LC412353 NLN HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF504327 Carrier-free (BSA/glycerol-free) NLN mouse monoclonal antibody, clone OTI3H4 (formerly 3H4) 100ug $450 3-4 weeks
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WB(2)
IHC(5)
FC(3)

OriGene Data

ImmunogenFull length human recombinant protein of human NLN(NP_065777) produced in HEK293T cell.
Clone NameClone OTI3H4 IsotypeIgG2b
Species ReactivityHuman , Dog , Rat , Monkey , Mouse Concentration0.92 mg/ml
Guaranteed Application *WB, IHC, FC Suggested Dilutions WB 1:500~2000, IHC 1:150, FLOW 1:100,
Predicted MW Explanation 80.5 kDa
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens neurolysin (metallopeptidase M3 family) (NLN)
Alternative NameAGTBP; EP24.16; MEP; MOP
Database LinkNP_065777
Entrez Gene 57486 Human
Entrez Gene 75805 Mouse
Entrez Gene 117041 Rat
Entrez Gene 478081 Dog
FunctionThis gene encodes a member of the metallopeptidase M3 protein family that cleaves neurotensin at the Pro10-Tyr11 bond, leading to the formation of neurotensin(1-10) and neurotensin(11-13). The encoded protein is likely involved in the termination of the neurotensinergic signal in the central nervous system and in the gastrointestinal tract.[provided by RefSeq, Jun
Related PathwayProteaseDruggable Genome Renin-angiotensin system

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NLN (RC212447, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NLN.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NLN monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504327)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504327)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504327)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504327)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-NLN mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA504327)
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HEK293T cells transfected with either RC212447 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NLN antibody(TA504327), and then analyzed by flow cytometry.
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Flow cytometric Analysis of Hela cells, using anti-NLN antibody(TA504327),(Red), compared to a nonspecific negative control antibody,(Blue).
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Flow cytometric Analysis of Jurkat cells, using anti-NLN antibody(TA504327),(Red), compared to a nonspecific negative control antibody,(Blue).
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