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Home Antibody All anti-NKX2 antibodies

Anti-NKX2 Antibody EP1584Y

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA300941
  • Rabbit Monoclonal Antibody against NKX2 (Clone EP1584Y)
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC401141) , 20ug Explanation
100ul 325 In Stock
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WB(2)
IHC(3)
IF(1)
FC(1)

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the N-terminus of human TTF1 was used as an immunogen.
Clone NameEP1584Y IsotypeIgG
Species ReactivityMouse, Rat, Human ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsICC/IF: Use at an assay dependent concentration; IHC-P: 1:100 - 1:250; WB: 1:500 - 1:1000; ICC: 1:100 - 1:250; FC: 1:30 - 1:1000; IHC-Fr: 1:400
BufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol, 0.05% BSA
Purification Tissue culture supernatant
Note Is unsuitable for IP.

Reference Data

Target NameHomo sapiens NK2 homeobox 1 (NKX2-1), transcript variant 2
Alternative NameBCH; BHC; NK-2; NKX2.1; NKX2A; TEBP; TITF1; TTF-1; TTF1
Database LinkNP_003308
Entrez Gene 7080 Human
Entrez Gene 21869 Mouse
Entrez Gene 25628 Rat
FunctionThe thyroid transcription factor 1 (TTF-1) is a homeodomain-containing transcription factor that activates the transcriptional activity of thyroid-specific gene promoters by binding to them. Hence, TTF-1 is crucial in the maintenance of the thyroid differentiation phenotype (1). Thyroid dysgenesis (TD) is responsible for most cases of congenital hypothyroidism, a condition that affects about one in 4000 newborns. Mutations in PAX8, TTF1, or FOXE1 may account for congenital hypothyroidism in patients with either isolated TD or TD with associated malformations involving kidney, lung, forebrain, and palate (2). In addition to its presence in thyroid gland epithelium, the human TTF-1 protein was detected by immunohistochemistry in human fetal lung as early as 11 weeks of gestation, being localized in the nuclei of epithelial cells of the developing airways. After birth, TTF-1 was selectively expressed in Type II epithelial cells in the alveoli and in subsets of bronchiolar epithelial cells in the conducting regions of the lung (3).
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
Western blot - TTF1 antibody [EP1584Y]; All lanes : Anti-TTF1 antibody [EP1584Y] at 1/2000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Mouse lung lysate.Lane 3 : Rat lung lysate.Lysates/proteins at 10 ug per lane.Secondary.Goat anti-rabbit HRP at 1/1000 dilution.developed using the ECL technique.Predicted band size : 38-42 kDa.Observed band size : 36,39 kDa .
WB Image
Western blot - Anti-TTF1 antibody [EP1584Y]; Anti-TTF1 antibody [EP1584Y] at 1/2000 dilution + HEK293 whole cell lysate at 30 ug.Secondary.HRP-conjugated goat anti-rabbit polyclonal IgG at 1/10000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 38-42 kDa.Observed band size : 40 kDa .Exposure time : 2 minutes
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TTF1 antibody [EP1584Y]; TA300941, at 1/100 dilution, staining TTF1 in human thyroid carcinoma by immunohistochemistry using paraffin-embedded tissue.
IHC Image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TTF1 antibody [EP1584Y]; TA300941, at 1/100 dilution, staining TTF1 in human lung adenocarcinoma by immunohistochemistry using paraffin-embedded tissue.
IHC Image
Immunohistochemistry (Frozen sections) - TTF1 antibody [EP1584Y]; TA300941 staining TTF1 in murine fetal lung tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in formaldehyde and permeabilized using PBST. Samples were then blocked using 1.5% serum for 20 minutes at 25°C, then incubated with TA300941 at a 1/400 dilution for 12 hours at 4°C. A biotin conjugated goat anti-rabbit IgG was used as the secondary at a 1/200 dilution.
IF Image
Immunocytochemistry/ Immunofluorescence - Anti-TTF1 antibody [EP1584Y]; Immunofluorescence analysis of rat bronchi cells, staining TTF1 with TA300941. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 27°C. Samples were incubated with primary antibody (1/500 in blocking buffer) for 1 hour at 16°C. An AlexaFluor?594-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.
FC Image
Flow Cytometry - Anti-TTF1 antibody [EP1584Y]; Overlay histogram showing A549 cells stained with TA300941 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1ug/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

 

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