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Anti-NKX2 Antibody EP1584Y
Also for NKX2 (NM_003317)
|A synthetic peptide corresponding to residues near the N-terminus of human TTF1 was used as an immunogen.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||ICC/IF: Use at an assay dependent concentration; IHC-P: 1:100 - 1:250; WB: 1:500 - 1:1000; ICC: 1:100 - 1:250; FC: 1:30 - 1:1000; IHC-Fr: 1:400
Preservative: 0.01% Sodium azide
Constituents: PBS, 50% Glycerol, 0.05% BSA
|Tissue culture supernatant (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens NK2 homeobox 1 (NKX2-1), transcript variant 2|
|BCH; BHC; NK-2; NKX2.1; NKX2A; TEBP; TITF1; TTF-1; TTF1|
|The thyroid transcription factor 1 (TTF-1) is a homeodomain-containing transcription factor that activates the transcriptional activity of thyroid-specific gene promoters by binding to them. Hence, TTF-1 is crucial in the maintenance of the thyroid differentiation phenotype (1). Thyroid dysgenesis (TD) is responsible for most cases of congenital hypothyroidism, a condition that affects about one in 4000 newborns. Mutations in PAX8, TTF1, or FOXE1 may account for congenital hypothyroidism in patients with either isolated TD or TD with associated malformations involving kidney, lung, forebrain, and palate (2). In addition to its presence in thyroid gland epithelium, the human TTF-1 protein was detected by immunohistochemistry in human fetal lung as early as 11 weeks of gestation, being localized in the nuclei of epithelial cells of the developing airways. After birth, TTF-1 was selectively expressed in Type II epithelial cells in the alveoli and in subsets of bronchiolar epithelial cells in the conducting regions of the lung (3). |
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Western blot - TTF1 antibody [EP1584Y]; All lanes : Anti-TTF1 antibody [EP1584Y] at 1/2000 dilution.Lane 1 : HeLa cell lysate.Lane 2 : Mouse lung lysate.Lane 3 : Rat lung lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat anti-rabbit HRP at 1/1000 dilution.developed using the ECL technique.Predicted band size : 38-42 kDa.Observed band size : 36,39 kDa .
Western blot - Anti-TTF1 antibody [EP1584Y]; Anti-TTF1 antibody [EP1584Y] at 1/2000 dilution + HEK293 whole cell lysate at 30 µg.Secondary.HRP-conjugated goat anti-rabbit polyclonal IgG at 1/10000 dilution.developed using the ECL technique.Performed under reducing conditions.Predicted band size : 38-42 kDa.Observed band size : 40 kDa .Exposure time : 2 minutesThis image is courtesy of an anonymous Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TTF1 antibody [EP1584Y]; ab76013, at 1/100 dilution, staining TTF1 in human thyroid carcinoma by immunohistochemistry using paraffin-embedded tissue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TTF1 antibody [EP1584Y]; ab76013, at 1/100 dilution, staining TTF1 in human lung adenocarcinoma by immunohistochemistry using paraffin-embedded tissue.
Immunohistochemistry (Frozen sections) - TTF1 antibody [EP1584Y]; ab76013 staining TTF1 in murine fetal lung tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in formaldehyde and permeabilized using PBST. Samples were then blocked using 1.5% serum for 20 minutes at 25°C, then incubated with ab76013 at a 1/400 dilution for 12 hours at 4°C. A biotin conjugated goat anti-rabbit IgG was used as the secondary at a 1/200 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-TTF1 antibody [EP1584Y]; Immunofluorescence analysis of rat bronchi cells, staining TTF1 with ab76013. Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 30 minutes at 27°C. Samples were incubated with primary antibody (1/500 in blocking buffer) for 1 hour at 16°C. An AlexaFluor?594-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody.
Flow Cytometry - Anti-TTF1 antibody [EP1584Y]; Overlay histogram showing A549 cells stained with ab76013 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.