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Anti-NIT2 TRUEMAB Antibody Clone OTI5A3

TrueMAB™ Antibodies - Made against Authentic Protein Antigens


Specifications Citations Customer Reviews Product Documents
SKU Description Amount Price Availability*  
TA501121 anti-NIT2 mouse monoclonal antibody, clone OTI5A3 (formerly 5A3) 100ul 325
In Stock
LC402761 NIT2 HEK293T cell transient overexpression lysate (as WB positive control) 20ug $50 In Stock
CF501121 Carrier-free (BSA/glycerol-free) NIT2 mouse monoclonal antibody, clone OTI5A3 (formerly 5A3) 100ug $450 3-4 weeks
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OriGene Data

ImmunogenFull length human recombinant protein of human NIT2 (NP_064587) produced in HEK293T cell.
Clone NameClone OTI5A3 IsotypeIgG2a
Species ReactivityHuman Concentration0.95 mg/ml
Guaranteed Application *WB, IHC, IF Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100,
Predicted MW Explanation 30.4kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens nitrilase family, member 2 (NIT2)
Alternative NameHEL-S-8a
Database LinkNP_064587
Entrez Gene 56954 Human
FunctionHas a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle
Related Pathway

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT2 (RC210660, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT2.

IHC Image
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501121, Dilution 1:50)

IF Image
Anti-NIT2 mouse monoclonal antibody (TA501121) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT2(RC210660).

IF Image
Immunofluorescent staining of HT29 cells using anti-NIT2 mouse monoclonal antibody (TA501121).


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