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Home Antibody All anti-NIT2 antibodies

Anti-NIT2 TRUEMAB Antibody clone 3D5

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA501097
  • anti-NIT2 mouse monoclonal antibody, clone 3D5
  • FREE positive control: HEK293T cell transient overexpression lysate (LC402761) , 20ug
100ul 325 In Stock
CF501097
  • Carrier-free (BSA/glycerol-free) NIT2 mouse monoclonal antibody, clone 3D5
100ug $450 3-4 weeks
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WB(1)
IHC(3)
IF(1)
FC(1)
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Also for NIT2 (NM_020202)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human NIT2 (NP_064587) produced in HEK293T cell.
Clone Nameclone 3D5 IsotypeIgG2b
Species ReactivityHuman Concentration0.79 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens nitrilase family, member 2 (NIT2)
Alternative NameHEL-S-8a
Database LinkNP_064587
FunctionHas a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle
Related Pathway

* Shipping is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT2 (RC210660, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT2.
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501097, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded Kidney tissue using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA501097, Dilution 1:50)
IHC Image
Immunohistochemical staining of paraffin-embedded liver tissue using anti-NIT2 mouse monoclonal antibody. (TA501098, Dilution 1:50)
IF Image
Anti-NIT2 mouse monoclonal antibody (TA501097) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT2(RC210660).
FC Image
HEK293T cells transfected with either pCMV6-ENTRY NIT2(RC210660)(Red) or empty vector control plasmid(Blue) were immunostained with anti-NIT2 mouse monoclonal(TA501097), and then analyzed by flow cytometry.

 

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