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Anti-NIT2 TRUEMAB Antibody Clone OTI3D5
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for NIT2 (NM_020202)
|Full length human recombinant protein of human NIT2 (NP_064587) produced in HEK293T cell.|
|WB, IHC, IF, FC
||WB 1:2000, IHC 1:50, IF 1:100, Flow 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens nitrilase family, member 2 (NIT2)|
Entrez Gene 56954 Human
|Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NIT2 (RC210660, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NIT2.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NIT2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501097, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501097, Dilution 1:50)
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-NIT2 mouse monoclonal antibody. (TA501098, Dilution 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min)
Anti-NIT2 mouse monoclonal antibody (TA501097) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NIT2(RC210660).
HEK293T cells transfected with either pCMV6-ENTRY NIT2(RC210660)(Red) or empty vector control plasmid(Blue) were immunostained with anti-NIT2 mouse monoclonal(TA501097), and then analyzed by flow cytometry.