Anti-NHEJ1 TrueMAB Antibody clone 1G7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

SKU	Description	Amount	Price	Availability*
TA502008	NHEJ1 (XLF) mouse monoclonal antibody, clone 1G7 FREE positive control: HEK293T cell transient overexpression lysate (LC403031) , 20ug	100ul	$325	In Stock
CF502008	Carrier-free (BSA/glycerol-free) NHEJ1 mouse monoclonal antibody, clone 1G7	100ug	$450	3-4 weeks
TA802519S	Purified GAPDH mouse monoclonal antibody (Loading control), clone 2D9	30ul	$100 $50*	In Stock

* Buy one primary antibody, get 50% off a GAPDH loading control antibody.

Also for NHEJ1 (NM_024782)

OriGene Data

Immunogen	Full length human recombinant protein of human NHEJ1 (NP_079058) produced in HEK293T cell.
Clone Name	clone 1G7	Isotype	IgG1
Species Reactivity	Human	Concentration	0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *	WB, IF, FC	Suggested Dilutions	WB 1:2000, IF 1:100, FLOW 1:100
Buffer	PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification	Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens nonhomologous end-joining factor 1 (NHEJ1)
Alternative Name	XLF
Database Link	NP_079058
Function	Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq].
Related Pathway

* Shipping is in business days

* OriGene provides validated application data and protocol, with money back guarantee.

WB Image

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NHEJ1 (RC203393, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NHEJ1.

IF Image

Anti-NHEJ1 mouse monoclonal antibody (TA502008) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NHEJ1(RC203393).

FC Image

Flow cytometric Analysis of Hela cells, using anti-NHEJ1 antibody(TA502008),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

FC Image

Flow cytometric Analysis of Jurkat cells, using anti-NHEJ1 antibody(TA502008),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

Conjugation Services
OriGene provides conjugation services of TrueMAB antibodies with the following molecules:

HRP
Biotin
PE
FITC
DyLight® 488
DyLight® 550
DyLight® 594
DyLight® 633

* DyLight is a trademark of Thermo-Fisher Scientific

Bulk Order
OriGene offers bulk sizes of TrueMAB antibodies in both the BSA/glycerol formulation and the carrier-free (BSA/glycerol-free) formulation.

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