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Home Antibody All anti-NHEJ1 antibodies

Anti-NHEJ1 TRUEMAB Antibody clone 3E6

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA502004
  • NHEJ1 (XLF) mouse monoclonal antibody, clone 3E6
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC403031) , 20ug
100ul 325 In Stock
CF502004
  • Carrier-free (BSA/glycerol-free) NHEJ1 mouse monoclonal antibody, clone 3E6
100ug $450 3-4 weeks
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WB(2)
IHC(8)
IF(1)
FC(2)
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Also for NHEJ1 (NM_024782)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenFull length human recombinant protein of human NHEJ1 (NP_079058) produced in HEK293T cell.
Clone Nameclone 3E6 IsotypeIgG1
Species ReactivityHuman Concentration0.41 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:500~2000, IHC 1:150, IF 1:100, FLOW 1:100
BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens nonhomologous end-joining factor 1 (NHEJ1)
Alternative NameXLF
Database LinkNP_079058
FunctionDouble-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq].
Related Pathway

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* OriGene provides validated application data and protocol, with money back guarantee.

WB Image
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NHEJ1 (RC203393, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NHEJ1.
WB Image
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NHEJ1 monoclonal antibody.
IHC Image
Immunohistochemical staining of paraffin-embedded Human Kidney tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502004)
IHC Image
Immunohistochemical staining of paraffin-embedded Human liver tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502004)
IHC Image
Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502004)
IHC Image
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502004)
IHC Image
Immunohistochemical staining of paraffin-embedded Human prostate tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502004)
IHC Image
Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502004)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymph node tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502004)
IHC Image
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-NHEJ1 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA, pH8.0, TA502004)
IF Image
Anti-NHEJ1 mouse monoclonal antibody (TA502004) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NHEJ1(RC203393).
FC Image
HEK293T cells transfected with either RC203393 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NHEJ1 antibody(TA502004), and then analyzed by flow cytometry.
FC Image
Flow cytometric Analysis of Hela cells, using anti-NHEJ1 antibody(TA502004),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).

 

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