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Anti-NHEJ1 TrueMAB Antibody clone 3B5
TrueMAB Antibodies - Made against Authentic Protein Antigens
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Also for NHEJ1 (NM_024782)
|Full length human recombinant protein of human NHEJ1 (NP_079058) produced in HEK293T cell.|
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IF, FC
||WB 1:500~2000, IF 1:100, FLOW 1:100
|PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.|
|Purified from mouse ascites fluids by affinity chromatography (Protein A or G Sepharose)
|Homo sapiens nonhomologous end-joining factor 1 (NHEJ1)|
|Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq]. |
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NHEJ1 (RC203393, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NHEJ1.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NHEJ1 monoclonal antibody.
Anti-NHEJ1 mouse monoclonal antibody (TA502002) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NHEJ1(RC203393).
HEK293T cells transfected with either RC203393 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NHEJ1 antibody(TA502002), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-NHEJ1 antibody(TA502002),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
Flow cytometric Analysis of Jurkat cells, using anti-NHEJ1 antibody(TA502002),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).