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Home Antibody All anti-NFYA antibodies

Anti-NFYA Antibody

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA319427
  • Rabbit polyclonal anti-NF-Y antibody
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC400893) , 20ug
100ul 325 3-7 Days
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WB(1)
IHC(1)
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Also for NFYA (NM_002505)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenNF-Y (A subunit specific) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Clone Name IsotypeIgG
Species Reactivityhuman Concentration90 mg/mL
Guaranteed Application *WB, IHC Suggested DilutionsELISA: 1:20,000 - 1:100,000, WB: 1:2,000 - 1:10,000, IHC: 1:400, Gel Shift: Use 2.0 uL
BufferNone
Note Nuclear transcription factor Y subunit alpha is a protein that in humans is encoded by the NFYA gene. The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. Anti-NF-Y is ideal for investigators involved in transcription factor research.

Reference Data

Target NameHomo sapiens nuclear transcription factor Y, alpha (NFYA), transcript variant 1
Alternative NameCBF-A; CBF-B; HAP2; NF-YA
Database LinkNP_002496
Function
Related Pathway
TGF Beta Signaling Pathway

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WB Image
Western Blot of Rabbit Anti-NF-Y (A Subunit) Antibody. Lane 1: CHO-7 cells were cultured in the absence (I) of cholesterol. Lane 2: CHO-7 cells were cultured in the presence (S) of cholesterol. Load: 35 µg per lane. Primary antibody: NF-YA antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP conjugated Goat-anti-Rabbit IgG [H&L] secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: NF-YA recognized as a doublet of ~ 42 kDa in size. Other band(s): none.
IHC Image
Immunohistochemistry of Rabbit anti-NF-Y (A Subunit) antibody. Tissue: human prostate tissue. Fixation: formalin fixed paraffin embedded. Antigen retrieval: not required. Primary antibody: NF-Y antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: NF-Y is nuclear. Staining: NF-Y subunit A as precipitated red signal with hematoxylin purple nuclear counterstain.

 

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