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Anti-NFKBIE Antibody EPR901(2)
Also for NFKBIE (NM_004556)
|A synthetic peptide corresponding to residues in human IkappaB epsilon was used as an immunogen.|
||Tissue culture supernatant
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:1000 - 1:10000; IP: 1:10 - 1:100; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
|Does not react with Mouse, Rat. Is unsuitable for Flow Cyt.|
|Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA (Protein A or G Sepharose)
|Is unsuitable for Flow Cyt.
|Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon (NFKBIE)|
|IkappaB epsilon, a novel member of the IkappaB family, has six consecutive ankyrin repeats. IkappaB epsilon interacts with different NF-kappaB proteins, including p65 (RelA), c-Rel, p50, and p52, in vitro and in vivo and inhibits the DNA-binding activity of both p50-p65 and p50-c-Rel complexes effectively. IkappaB epsilon displays differential induction in response to tumor necrosis factor alpha, interleukin-1, or phorbol ester stimulation compared to I kappaB alpha in non-B-cell lines. This protein provides an alternative mechanism for regulation of NF-kappaB-dependent transcription (1).|
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Western blot - Anti-IKB epsilon antibody [EPR901(2)]; All lanes : Anti-IKB epsilon antibody [EPR901(2)] at 1/1000 dilution.Lane 1 : THP-1 cell lysate.Lane 2 : Jurkat cell lysate.Lane 3 : HeLa cell lysate.Lane 4 : A375 cell lysate.Lysates/proteins at 10 µg per lane.Secondary.Goat Anti-rabbit HRP at 1/2000 dilution.Predicted band size : 53 kDa.Observed band size : 45 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB epsilon antibody [EPR901(2)]; Immunohistochemical analysis of Paraffin-embedded Human tonsil tissue labelling IKB epsilon with ab134143 at 1/100 dilution.