Anti-NFKBIA Antibody, EPR3148

Also for NFKBIA (NM_020529)

OriGene Data

Immunogen	A phospho specific peptide corresponding to residues surrounding Serine 32 of human IkappaB-alpha was used as an immunogen.
Clone Name	EPR3148	Isotype	Rabbit IgG
Species Reactivity	Human	Concentration
Guaranteed Application *	WB	Suggested Dilutions	ICC: 1:50 - 100, IP: 1:10 - 100, WB: 1:1,000 - 10,000,
Buffer	50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Purification	(Protein A or G Sepharose)

Reference Data

Target Name	Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA)
Alternative Name	IKBA; MAD-3; NFKBI
Database Link	NP_065390
Function	The NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB-alpha protein (1-5). In response to many different NF-kappaB-inducing agents including T-cell mitogens, proinflammatory cytokines, and viral transactivators, the inhibitory IkappaB-alpha is rapidly phosphorylated and degraded (2). Phosphorylation of IkappaB-alpha at Ser32 and Ser36 has been shown to stimulate conjugation with ubiquitin and subsequent degradation of IkappaB-alpha (3). Activation of NF-kappaB can also be achieved after tyrosine phosphorylation of IkappaBalpha at tyrosine 42, an event that also ultimately leads to the dissociation of NF-kappaB and IkappaBalpha (4). Phosphorylation of IkappaB-alpha at Ser32 and Tyr42 is critical for activation of NF-kappaB, which then translocates to the nucleus and induces transcription of genes that protect organism (1-5).
Related Pathway	Apoptosis Delta-Notch Signaling Pathway Toll-like receptor signaling pathway

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WB Image

Western blot analysis on Hela cell lysates using anti-Phospho-IkappaB-alpha (pS32) RabMAb .

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Anti-NFKBIA Phospho Antibody EPR3148

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

OriGene Data

Reference Data