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Home Antibody All anti-NFKBIA antibodies

Anti-NFKBIA PHOSPHO Antibody EPR2353

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Specifications Citations Related Products Product Documents
SKU Description Amount Price Availability*  
TA307067
  • Rabbit monoclonal antibody against IkappaB-alpha Phospho (pY42) (clone EPR2353 ) (Phospho-Specific)
100ul 325 3-7 Days
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WB(1)
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Also for NFKBIA (NM_020529)
cDNA Clone shRNA/siRNA Lysate Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues surrounding Tyrosine 42 of human IKappaB-alpha was used as an immunogen.
Clone NameEPR2353 IsotypeIgG
Species ReactivityHuman Concentration0.5~1.0 mg/ml (Lot Dependent)
Guaranteed Application *WB Suggested DilutionsIP: 1:10, WB: 1:500 - 1,000,
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purification Tissue culture supernatant
Note Does not react with Mouse, Rat

Reference Data

Target NameHomo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA)
Alternative NameIKBA; MAD-3; NFKBI
Database LinkNP_065390
FunctionThe NF-kappaB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IkappaB-alpha protein (1-5). In response to many different NF-kappaB-inducing agents including T-cell mitogens, proinflammatory cytokines, and viral transactivators the inhibitory IkappaB-alpha is rapidly phosphorylated and degraded (2). Phosphorylation of IkappaB-alpha at Ser32 and Ser36 has been shown to stimulate conjugation with ubiquitin and subsequent degradation of IkappaB-alpha (3). Activation of NF-kappaB can also be achieved after tyrosine phosphorylation of IkappaBalpha at tyrosine 42, an event that also ultimately leads to the dissociation of NF-kappaB and IkappaBalpha (4). Phosphorylation of IkappaB-alpha at Ser32 and Tyr42 is critical for activation of NF-kappaB, which then translocates to the nucleus and induces transcription of genes that protect organism (1-5).
Related Pathway
Apoptosis
Delta-Notch Signaling Pathway
Toll-like receptor signaling pathway

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WB Image
Western blot - IKB alpha (phospho Y42) antibody [EPR2353]; All lanes : Anti-IKB alpha (phospho Y42) antibody [EPR2353] at 1/500 dilution.Lane 1 : lysate from untreated A431 cells.Lane 2 : lysate from A431 cells pre-treated with pervanadate.Lysates/proteins at 10 µg per lane.Secondary.HRP conjugated Goat anti-Rabbit IgG at 1/2000 dilution.Predicted band size : 36 kDa.

 

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