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Anti-NFKB1 Antibody E381
Also for NFKB1 (NM_003998)
|A synthetic peptide corresponding to residues in the nuclear factor NF-?B p50 subunit was used as immunogen. This antibody will detect both forms; p50 and p105.|
|Human, Mouse, Rat
||0.5~1.0 mg/ml (Lot Dependent)
|WB, IHC, IF, FC
||WB: 1:1000 - 1:10000; IHC-P: 1:250 - 1:500; ICC: 1:250 - 1:500; FC: 1:30
|PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%|
|Protein A purified (Protein A or G Sepharose)
|Is unsuitable for IP.
|Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1), transcript variant 1|
|EBP-1; KBF1; NF-kappa-B; NF-kappaB; NF-kB1; NFkappaB; NFKB-p105; NFKB-p50; p105; p50|
|This gene encodes a 105 kD protein which can undergo cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of the NF-kappa-B (NFKB) protein complex. NFKB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products. Activated NFKB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFKB has been associated with a number of inflammatory diseases while persistent inhibition of NFKB leads to inappropriate immune cell development or delayed cell growth. [provided by RefSeq]. |
MAPK signaling pathway
TGF Beta Signaling Pathway
Toll-like receptor signaling pathway
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Western blot - NFkB p105 / p50 antibody [E381]; Anti-NFkB p105 / p50 antibody [E381] at 1/5000 dilution + HeLa cell lysate.Predicted band size : 50 kDa.Observed band size : 50,105 kDa .
Immunohistochemistry (Paraffin-embedded sections) - NFkB p105 / p50 antibody [E381]; Paraffin-embedded human prostate carcinoma.ab32360 at 1/250 dilution
Immunocytochemistry/ Immunofluorescence - NFkB p105 / p50 antibody [E381]; ICC/IF image of ab32360 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody overnight at +4°C. The secondary antibody (green) was Alexa Fluor? 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor? 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Flow Cytometry-NFkB p105 / p50 antibody [E381](ab32360); Overlay histogram showing K562 cells stained with ab32360 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody for 30 min at 22°C. The secondary antibody used was DyLight? 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in K562 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween used under the same conditions.