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Home Antibody All anti-NFE2L2 antibodies

Anti-NFE2L2 Antibody EP1808Y

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Specifications Citations (2) Related Products Product Documents
SKU Description Amount Price Availability*  
TA303616 Rabbit monoclonal antibody against Nrf2 (EP1808Y ) 100ul $325 In Stock
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WB(3)
IHC(1)
IF(2)
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Also for NFE2L2 (NM_006164)
cDNA Clone shRNA/siRNA CRISPR KO Kit Protein Antibody

OriGene Data

ImmunogenA synthetic peptide corresponding to residues near the C-terminus of human Nrf2 was used as an immunogen.
Clone NameEP1808Y IsotypeIgG
Species ReactivityHuman (Does not react with: Rat) ConcentrationLot dependent; please refer to CoA along with shipment
Guaranteed Application *WB, IHC, IF Suggested DilutionsIHC-Fr: 1:100; ICC/IF: Use at an assay dependent dilution; WB: 1:1000 - 1:5000; IP: 1:20; IHC-P: 1:100 - 1:250; ICC: 1:100 - 1:250
BufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Note Is unsuitable for Flow Cyt.

Reference Data

Target NameHomo sapiens nuclear factor, erythroid 2-like 2 (NFE2L2), transcript variant 1
Alternative NameNRF2
Database LinkNP_006155
Entrez Gene 4780 Human
FunctionNFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM].
Related PathwayTranscription Factors

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

Western blot - Nrf2 antibody [EP1808Y] - Carboxyterminal end; Anti-Nrf2 [EP1808Y] antibody at 1/2000 dilution + HepG2 cell lysate at 10 ug.Secondary.goat anti rabbit HRP at 1/2000 dilution.Predicted band size : 68 kDa.Observed band size : 100 kDa .
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Figure from citation: Western Blot of NFE2L2(Nrf2) protein level by using anti-NFE2L2 antibody in human A549 cells. View Citation
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Figure from citation: Western Blot of NFE2L2(Nrf2) protein level by using anti-NFE2L2 antibody in brain cortex from rat embryos. View Citation
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Nrf2 antibody [EP1808Y] - Carboxyterminal end; Immunohistochemical analysis of Nrf2 expression in paraffin embedded human lung adenocarcinoma using 1/100 TA303616
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ICC/IF image of TA303616 stained Hek293 cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was Alexa Fluor 488 goat anti-rabbit IgG (H+L) used at 1:1000 for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
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ICC/IF image of TA303616 stained HeLa cells. The cells were incubated with the antibody overnight at 4.. The secondary antibody (green) was DyLight 488 goat anti-rabbit IgG - H&L, pre-adsorbed used at 1:250. for 1h. Alexa Fluor 594 WGA was used to label plasma membranes (red) at 1:200 for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43uM.
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