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Anti-NEUROG3 TRUEMAB Antibody Clone OTI3B5
TrueMAB Antibodies - Made against Authentic Protein Antigens
Also for NEUROG3 (NM_020999)
|Full-length protein expressed in 293T cell transfected with human NEUROG3 expression vector|
|WB, IHC, IF, IP
||WB 1:2000, IHC 1:50 - 1:150, IF 1:100
|PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide|
|Purified from mouse ascites fluids by affinity chromatography
|Homo sapiens neurogenin 3 (NEUROG3)|
|Atoh5; bHLHa7; Math4B; NGN-3; ngn3|
Entrez Gene 50674 Human
|Neurogenin-3 (NEUROG3) is expressed in endocrine progenitor cells and is required for endocrine cell development in the pancreas and intestine (Wang et al., 2006 [PubMed 16855267]). It belongs to a family of basic helix-loop-helix transcription factors involved in the determination of neural precursor cells in the neuroectoderm |
|ES Cell Differentiation/IPS Maturity onset diabetes of the young|
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEUROG3 (RC224767, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEUROG3.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NEUROG3 mouse monoclonal antibody (TA500346) at 1:150 dilution.
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-NEUROG3 mouse monoclonal antibody (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min).
Anti-NEUROG3 mouse monoclonal antibody (NEUROG3) immunofluorescent staining of HeLa cells transiently transfected by pCMV6-ENTRY NEUROG3 (RC224767).
Immunoprecipitation of NEUROG3 by using TrueMab monoclonal anti-NEUROG3 antibody (Negative control: IP without adding anti-NEUROG3 antibody). For each experiment, 500ul of DDK tagged NEUROG3 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2u