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Home Antibody All anti-NEK6 antibodies

Anti-NEK6 TRUEMAB Antibody clone 5D7

TrueMAB™ Antibodies - Made against Authentic Protein Antigens

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Specifications Citations (0) Related Products Product Documents
SKU Description Amount Price Availability*  
TA500545
  • NEK6 mouse monoclonal antibody, clone 5D7
  • Free Sample of Positive Control: HEK293T cell transient overexpression lysate (LC402327) , 20ug Explanation
100ul $325 In Stock
CF500545
  • Carrier-free (BSA/glycerol-free) NEK6 mouse monoclonal antibody, clone 5D7
100ug $450 3-4 weeks
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WB(1)
IHC(8)
IF(1)
FC(1)

OriGene Data

ImmunogenFull length human recombinant protein of human NEK6 (NP_055212) produced in HEK293T cell.
Clone Nameclone 5D7 IsotypeIgG1
Species ReactivityHuman Concentration0.75 mg/ml
Guaranteed Application *WB, IHC, IF, FC Suggested DilutionsWB 1:2000, IHC 1:50, IF 1:100, FLOW 1:100,
Predicted MW Explanation 35.5 kDa
BufferPBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Purification Purified from mouse ascites fluids by affinity chromatography

Reference Data

Target NameHomo sapiens NIMA-related kinase 6 (NEK6), transcript variant 2
Alternative NameSID6-1512
Database LinkNP_055212
Entrez Gene 10783 Human
FunctionThe Aspergillus nidulans 'never in mitosis A' (NIMA) gene encodes a serine/threonine kinase that controls initiation of mitosis. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA.[supplied by OMIM].
Related PathwayProtein KinaseDruggable Genome

* Availability is in business days
* OriGene provides validated application data and protocol, with money back guarantee.

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEK6 (RC203609, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NEK6.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500545)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500545)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500545)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500545)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500545)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500545)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500545)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-NEK6 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500545)
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Anti-NEK6 mouse monoclonal antibody (TA500545) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NEK6(RC203609).
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HEK293T cells transfected with either RC203609 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-NEK6 antibody(TA500545), and then analyzed by flow cytometry.
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