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Also for NEK2 (NM_001204182)
|This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 287-299 of Human NEK2 protein.|
||ELISA: 1:2,000 - 1:10,000, WB: 1:500 - 1:2,000, IF: User Optimized
|0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2|
|NEK2, also called serine/threonine-protein kinase Nek2NimA-related protein kinase 2, NimA-like protein kinase 1 and HSPK 21, is a protein kinase involved in mitotic regulation that may have a role at the G2-M transition and may also play a role in meiosis. NEK2 has a nuclear localization and accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins.
|Homo sapiens NIMA-related kinase 2 (NEK2), transcript variant 3|
|HsPK21; NEK2A; NLK1; PPP1R111; RP67|
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Western blot analysis is shown using Affinity Purified anti-NEK2 antibody to detect endogenous protein present in an unstimulated mouse A-20 whole cell lysate (arrowhead). Comparison to a molecular weight marker indicates a band of ~52 kDa correspond-ing to NEK2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques. Personal communication Steven Pelech, Kinexus Inc.