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Anti-NEFM PHOSPHO Antibody EPR580(2)Y
Also for NEFM (NM_005382)
|A synthetic phospho-peptide corresponding to residues surrounding Serine 614/619 of human NF-M was used as an immunogen.|
||0.5~1.0 mg/ml (Lot Dependent)
||WB: 1:500; IHC: 1:100 - 250; IP: 1:30
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol, 0.05% BSA
|Tissue culture supernatant
|Homo sapiens neurofilament, medium polypeptide (NEFM), transcript variant 1|
|NEF3; NF-M; NFM|
|Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]. |
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Western blot - Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y]; All lanes : Anti-160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y] at 1/500 dilution.Lane 1 : SH-SY5Y cell lysate treated with Alkaline phosphatase.Lane 2 : Untreated SH-SY5Y cell lysate.Lysates/proteins at 10 µg per lane.Secondary.HRP conjugated Goat anti-rabbit at 1/2000 dilution.Predicted band size : 102 kDa.Observed band size : 160 kDa .
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 160 kD Neurofilament Medium (phospho S614 + S619) antibody [EPR580(2)Y]; Immunohistochemical analysis of paraffin-embedded human brain tissue using TA303832 at a dilution of 1/100 - 1/250